Biochemistry Notes > Queen Mary, University Of London Biochemistry Notes > Genes and Bioinformatics Notes

Prokaryote Transcription Notes

This is a sample of our (approximately) 3 page long Prokaryote Transcription notes, which we sell as part of the Genes and Bioinformatics Notes collection, a First (78%) package written at Queen Mary, University Of London in 2013 that contains (approximately) 37 pages of notes across 19 different documents.

Learn more about our Genes and Bioinformatics Notes

Prokaryote Transcription Revision

The following is a plain text extract of the PDF sample above, taken from our Genes and Bioinformatics Notes. This text version has had its formatting removed so pay attention to its contents alone rather than its presentation. The version you download will have its original formatting intact and so will be much prettier to look at.

Prokaryote Transcription: Transcription performed by RNA polymerase. mRNA produced is identical to 5'  3' nontemplate/coding strand (except U replaces T), and complementary to 3'  5' template strand. 2 main differences in transcription in prokaryotes and eukaryotes:

• Prokaryote transcription and translation occur at the same time, separate in eukaryotes due to presence of nucleus.

• Eukaryote mRNA undergoes post-translational processing, not in prokaryotes. RNA polymerase separates two strands of DNA in a transient "bubble". Uses one strand as a template to direct synthesis of complementary RNA sequence. It catalyses phosphodiester bond formation between adjacent nucleotides. Transcription has three stages: First RNA polymerase binds to a promoter site on DNA to form a closed complex.

1. Initiation: RNA polymerase initiates transcription after opening DNA duplex to form a transcription bubble (open complex).

2. Elongation: During elongation the transcription bubble moves along DNA. RNA chain extended in the 5′  3′ direction by adding nucleotides to 3' end.

3. Termination: When transcription terminates or stops the DNA duplex reforms, and RNA polymerase dissociates at a terminator site.

****************************End Of Sample*****************************

Buy the full version of these notes or essay plans and more in our Genes and Bioinformatics Notes.