Sanger Sequencing Notes

Updated Sanger Sequencing Notes

Biochemistry

Approximately 28 pages

This includes a set of detailed but concise notes on glycogen metabolism, fats transport, nitrogen transport, principles of glycogen metabolism, control of gene expression, synthesis of DNA vs RNA, DNA suitability, factors in genetic disease incidence, regulatory principles in metabolism, methods of genetic analysis, Sanger sequencing, the respiratory chain, the ornithine/urea cycle, and fatty acid metabolism. There are also two essays on what determines protein structure and how enzymes are suit...

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Sanger sequencing

Sanger sequencing is a method of sequencing the order of bases in DNA. It relies on the principle of controlled termination of replication. This is achieved by carrying out DNA replication in an experiment where a small amount one of the bases is radiolabelled, and altered such that when it is incorporated, it will terminate replication. Most of this base added will be normal, however some will be radiolabelled and if the experiment is done with enough molecules, DNA sequences of many different lengths will be synthesised, all of which will have a final radiolabelled base of a specific type, adenine, guanine, thymine or cytosine. This experiment is repeated four times, once for each base....

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