Veterinary Medicine Notes > Universiti Putra Malaysia Veterinary Medicine Notes > Common Bacterial Infections in Chickens - Veterinary Bacteriology Notes
Common Bacterial Infections In Chickens Notes
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Colibacillosis or colisepticem ia in poultry
Prevention, tx n con
Systemic disease - bacteremia (acute septicemia or subacute airsacculitis
&polyserositis in young broilers).
Postmortem lesions - hepatitis &splentitis, with increased fluids accumulations in the thoracic and abdominal cavities.
•Survivors of acute septicemia usually develop pericarditis, perihepatitis&
•Other less common disease manifestation - pneumonia, salpingitis, arthritis &
Samples Septicemic disease - heart blood, bone marrow &parenchymatous organs Isolation of E. coli Media - tryptose blood agar
&MacConkey agar Incubation - aerobic, 37ºC for 24 to 48 hours Colonies on blood agar - smooth or rough (2-3mm in diameter), low convex, moist, shiny, entire & grey) Colonies on MacConkey agar - lactose positive Identification of E. coli Tube biochemical or commercial strip test. (TSI, SIM, Oxidase, catalase, nitrate reduction) Molecular detection method - PCR to detect virulence factors genes
•Antibiotic susceptibility tes
•Amoxycillin, tetracyclines, neomycin (intestinal activit gentamycin or ceftiofur, po sulphonamide, flouroquinol
•Good hygiene in handling hatching eggs, hatchery hy good sanitation of house, fe water. Well-nourished embr optimal incubation to maxim old viability.
•Control of predisposing fac infections (usually by vaccin
Pullorum disease (bacillary white diarrhoea )
-Chicken (aftr 2w hacthing)
-Route of infection is oral or via the navel/yolk. Septicemia, highly fatal to eggs and chicks
-Birds huddle together &
exhibit a white foamy diarrhea. Adult birds loss of appetite, weakness, greenish brown diarrhea
-The mortality rate is high and affected birds anorexic, depressed and have whitish fecal pasting around their vents.
•Characteristic lesions include whitish nodes throughout the lungs and focal necrosis of liver and spleen.
-Inappetance, depression, ruffled feathers, closed eyes, loud chirping, white diarrhea, vent pasting, gasping, lameness
Samples GIT disease - intestinal contents Septicemic disease - heart blood Isolation of Salmonella spp Media - Rappaport-Vassiliadis medium, xylose lysine deoxycholate (XLD) agar, MacConkey agar. Incubation - aerobic, 37ºC for 24 to 48 hours Colonies on XLD- pink colonies with black centre/completely black Colonies on MacConkey agar - non lactose fermenting Identification of Salmonella spp Tube biochemical or commercial strip test. (TSI, SIM, Oxidase, catalase, nitrate reduction) Molecular identification method
- PCR targeting Salmonella-specific genes.
Antibiotic Amoxycillin, tetracycline, fluoroquinolones (pullorum disease in chicken)
Innate immune response important in controlling the phases of infection with Salmonella spp Eradication
•can be largely eradicated infected adult birds are slaughtered.
•good hatchery hygiene.
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