This is an extract of our Common Bacterial Infections In Chickens document, which we sell as part of our Common Bacterial Infections in Chickens - Veterinary Bacteriology Notes collection written by the top tier of Universiti Putra Malaysia students.
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Colibacillosis or colisepticem ia in poultry
Prevention, tx n con
Systemic disease - bacteremia (acute septicemia or subacute airsacculitis
&polyserositis in young broilers).
Postmortem lesions - hepatitis &splentitis, with increased fluids accumulations in the thoracic and abdominal cavities.
*Survivors of acute septicemia usually develop pericarditis, perihepatitis&
*Other less common disease manifestation - pneumonia, salpingitis, arthritis &
Samples Septicemic disease - heart blood, bone marrow &parenchymatous organs Isolation of E. coli Media - tryptose blood agar
&MacConkey agar Incubation - aerobic, 37oC for 24 to 48 hours Colonies on blood agar - smooth or rough (2-3mm in diameter), low convex, moist, shiny, entire & grey) Colonies on MacConkey agar - lactose positive Identification of E. coli Tube biochemical or commercial strip test. (TSI, SIM, Oxidase, catalase, nitrate reduction) Molecular detection method - PCR to detect virulence factors genes
*Antibiotic susceptibility tes
*Amoxycillin, tetracyclines, neomycin (intestinal activit gentamycin or ceftiofur, po sulphonamide, flouroquinol
*Good hygiene in handling hatching eggs, hatchery hy good sanitation of house, fe water. Well-nourished embr optimal incubation to maxim old viability.
*Control of predisposing fac infections (usually by vaccin
Pullorum disease (bacillary white diarrhoea )
-Chicken (aftr 2w hacthing)
-Route of infection is oral or via the navel/yolk. Septicemia, highly fatal to eggs and chicks
-Birds huddle together &
exhibit a white foamy diarrhea. Adult birds loss of appetite, weakness, greenish brown diarrhea
-The mortality rate is high and affected birds anorexic, depressed and have whitish fecal pasting around their vents.
*Characteristic lesions include whitish nodes throughout the lungs and focal necrosis of liver and spleen.
-Inappetance, depression, ruffled feathers, closed eyes, loud chirping, white diarrhea, vent pasting, gasping, lameness
Samples GIT disease - intestinal contents Septicemic disease - heart blood Isolation of Salmonella spp Media - Rappaport-Vassiliadis medium, xylose lysine deoxycholate (XLD) agar, MacConkey agar. Incubation - aerobic, 37oC for 24 to 48 hours Colonies on XLD- pink colonies with black centre/completely black Colonies on MacConkey agar - non lactose fermenting Identification of Salmonella spp Tube biochemical or commercial strip test. (TSI, SIM, Oxidase, catalase, nitrate reduction) Molecular identification method
- PCR targeting Salmonella-specific genes.
Antibiotic Amoxycillin, tetracycline, fluoroquinolones (pullorum disease in chicken)
Innate immune response important in controlling the phases of infection with Salmonella spp Eradication
*can be largely eradicated infected adult birds are slaughtered.
*good hatchery hygiene.
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