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Dermatological Sampling And Practical Techniques Notes

Veterinary Medicine Notes > Endocrinology and Integument 2 Notes

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Dermatological sampling and practical techniques

1. Coat brushing/combing Place debris from coat brushings onto moistened paper to examineFlea dirt.

Examine coat combings in liquid paraffin under a coverslip under the microscope.Cheyletiella mites or eggs

2. Acetate tape strip (unstained) Used to sample for Cheyletiella or for lice. The tape is pressed over multiple sites of scaling and dragged across the hairshafts. It is then placed directly on the slide without staining.

3. Skin scrapings Skin scrapes may be deep or superficial

Superficial skin scrapes are diagnostic for surface parasites such as Cheyletiella, Neotrombicula and Otodectes, and for dermatophyte spore infection of hair shafts. Deep skin scrapes are required for deeper parasites such as Demodex and Sarcoptes. The hair should be clipped from around the area to be sampled. Mineral oil is placed onto a scalpel blade and a few drops placed on the skin. The skin should be squeezed prior to sampling. The blade is scraped over the skin surface at a 90 degree angle to the skin in the direction of the hair growth. For a superficial scrape, there is no need to draw blood. For a deep skin scrape, keep going until blood is seen. The material is deposited on one or more slides in mineral oil and covered with a coverslip.

4. Trichograms Trichogram involves plucking hair. The hairs are suspended in liquid paraffin and examined under a coverslip. The hairs should be plucked near their base with fine forceps in the direction of hair growth. Telogen bulbs are spear shaped and rough. These are inactive hairs and are normally present in around 80-90%. In endocrinopathies, 100% of hairs will be in telogen.

Anagen bulbs are rounded and smooth. They are actively growing hairs and usually account for 10-20%.

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